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| From Peptide Sequences Provided by Customers To Protein A/G purified total IgGs (PS-AG) |
Production Description |
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| Step 1) Peptide synthesis at AbMART | |||||
| Step 2) Peptides conjugated to KLH for immunization and to BSA for ELISA | |||||
| Step 3) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate | |||||
| Step 4) ELISA monitoring of anti-sera development until titers reach >10,000 | |||||
| Step 5) Protein A/G purification of production bleed |
| Order Today To Follow This Production Schedule | ||||||
Date |
Day |
AbMART Track |
Production Steps
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01-06-2009 |
1 |
Order Date |
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01-26-2009 |
18 |
Peptide Synthesis |
Peptide synthesis based on sequences provided by customers;
Conjugation to KLH (for immunization) and to BSA (for ELISA); Two rabbits ready (take pre-bleeds) |
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01-26-2009 |
21 |
Peptide Receive |
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01-27-2009 |
22 |
Peptide Conjugation |
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01-28-2009 |
23 |
Adjuvant Addition |
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01-29-2009 |
24 |
Production Start |
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01-29-2009 |
24 |
IMM-1 |
1st Immunization (Complete Freud Adjuvant)
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02-26-2009 |
52 |
IMM-2 |
2nd Immunization (Incomplete Freud Adjuvant)
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03-12-2009 |
66 |
IMM-3 |
3rd Immunization (Incomplete Freud Adjuvant)
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03-19-2009 |
73 |
BLD-P1 |
Bleed; ELISA
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After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
|
Date |
03-27-2009 |
03-26-2009 |
80 |
IMM-4 |
4th Immunization (Incomplete Freud Adjuvant)
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04-05-2009 |
90 |
BLD-P2 |
Bleed; ELISA
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04-07-2009 |
92 |
BLD-F1 |
Final Bleed
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04-10-2009 |
95 |
Purify Antibody |
Protein A/G purification of production bleed |
Day |
81 |
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04-15-2009 |
100 |
Shipment |
1 mg of synthetic peptide |
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1 ml Pre-bleed from each rabbit |
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>100 mg of total IgGs from each rabbit
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ELISA data
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