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| From Peptide Sequences Provided by Customers To Anti-sera (PS-AS) |
Production Description |
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| Step 1) Peptide synthesis at AbMART | |||||
| Step 2) Peptides conjugated to KLH for immunization and to BSA for ELISA | |||||
| Step 3) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate | |||||
| Step 4) ELISA monitoring of anti-sera development until titers reach >10,000 | |||||
| Step 5) 30-50 ml of anti-sera from production bleed of each rabbit |
Order Today To Follow This Production Schedule |
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Date
|
Day
|
AbMART Track
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Production Steps
|
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11-21-2008
|
1
|
Order Date
|
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12-11-2008 |
18 |
Peptide Synthesis |
Peptide synthesis based on sequences provided by customers; Conjugation to KLH (for immunization) and to BSA (for ELISA); Two rabbits ready (take pre-bleeds) |
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12-11-2008
|
21
|
Peptide Receive
|
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12-12-2008
|
22
|
Peptide Conjugation
|
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12-13-2008
|
23
|
Adjuvant Addition
|
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12-14-2008
|
24
|
Production Start
|
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12-14-2008 |
24
|
IMM-1
|
1st Immunization (Complete Freud Adjuvant) |
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01-11-2009 |
52
|
IMM-2
|
2nd Immunization (Incomplete Freud Adjuvant) |
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01-25-2009 |
66
|
IMM-3
|
3rd Immunization (Incomplete Freud Adjuvant) |
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02-01-2009 |
73
|
BLD-P1
|
Bleed; ELISA |
After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as | Date |
02-06-2009 |
02-08-2009 |
80 |
IMM-4
|
4th Immunization (Incomplete Freud Adjuvant) |
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02-18-2009 |
90 |
BLD-P2
|
Bleed; ELISA |
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02-20-2009 |
92 |
BLD-F1
|
Final Bleed |
Day |
78 |
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02-18-2009 |
97 |
Shipment
|
1 ml Pre-bleed from each rabbit |
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30-50 ml of anti-sera from each rabbit |
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ELISA data |
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