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From Protein Sequences Provided by Customers To Protein A/G Purified Total IgGs (PD-AG)               

 Production Description
Step 1) Peptide synthesis at AbMART
Step 2) Peptides conjugated to KLH for immunization and to BSA for ELISA
Step 3) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate
Step 4) ELISA monitoring of anti-sera development until titers reach >10,000
Step 5) Protein A/G affinity purification of production bleed

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
11-21-2008
1
Order Date
 
11-23-2008
3
Peptide Design
 Peptide synthesis based on sequences provided by customers;
Conjugation to KLH (for immunization) and to BSA (for ELISA);
Two rabbits ready (take pre-bleeds)
12-10-2008
20
Peptide Synthesis
12-13-2008
23
Peptide Receive
12-14-2008
24
Peptide Conjugation
12-15-2008
25
Adjuvant Addition
12-16-2008
26
Production Start
12-16-2008
26
IMM-1
1st Immunization (Complete Freud Adjuvant)
01-13-2009
54
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
01-27-2009
68
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
02-03-2009
75
BLD-P1
Bleed; ELISA
 After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
02-11-2009
02-10-2009
82
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
02-20-2009
92
BLD-P2
Bleed; ELISA
02-22-2009
94
BLD-F1
Final Bleed
Day
83
02-25-2009
97
Purify Antibody
 Protein A/G purification of production bleed
03-02-2009
102
Shipment

1 mg of synthetic peptide
1 ml Pre-bleed from each rabbit
>100 mg of total IgGs from each rabbit
ELISA data


2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn