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 From Protein Sequences Provided by Customers To Peptide-affinity Purified IgGs (PD-AP)               

 Production Description
Step 1) Peptide synthesis at AbMART
Step 2) Peptides conjugated to KLH for immunization and to BSA for ELISA
Step 3) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate
Step 4) ELISA monitoring of anti-sera development until titers reach >10,000
Step 5) Peptide-affinity purification of production bleed

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
01-06-2009
1
Order Date
 
01-08-2009
3
Peptide Design

Peptide design and synthesis based on protein sequences provided by customers;
Conjugation to KLH (for immunization) and to BSA (for ELISA);
Two rabbits ready (take pre-bleeds)
01-25-2009
20
Peptide Synthesis
01-28-2009
23
Peptide Receive
01-29-2009
24
Peptide Conjugation
01-30-2009
25
Adjuvant Addition
01-31-2009
26
Production Start
01-31-2009
26
IMM-1
1st Immunization (Complete Freud Adjuvant)
02-28-2009
54
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
03-14-2009
68
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
03-21-2009
75
BLD-P1
Bleed; ELISA
 After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
03-29-2009
03-28-2009
82
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
04-07-2009
92
BLD-P2
Bleed; ELISA
04-09-2009
94
BLD-F1
Final Bleed
Day
83
04-12-2009
97
Purify Antibody
 Protein A/G purification of production bleed
04-17-2009
102
Shipment

1 mg of synthetic peptide
1 ml Pre-bleed from each rabbit
1-5 mg of antigen-specific IgGs from each rabbit
ELISA data

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn