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From cDNA Clones Provided by Customers To Protein A/G purified total IgGs (CD-AG)            

 Production Description
Step 1) Recombinant protein production at AbMART
Step 2) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate
Step 3) ELISA monitoring of anti-sera development until titers reach >10,000
Step 4) Protein A/G affinity purification of production bleed

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
01-06-2009
1
Order Date
 
01-09-2009
4
Peptide Design

Design gene cloning strategy for expression plasmid vector construction;
Recombinant protein production and purification;
Two rabbits ready (take pre-bleeds)
02-13-2009
39
Peptide Synthesis
02-16-2009
42
Peptide Receive
02-16-2009
43
Adjuvant Addition
02-18-2009
44
Production Start
02-18-2009
44
IMM-1
1st Immunization (Complete Freud Adjuvant)
03-18-2009
72
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
04-01-2009
86
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
04-08-2009
93
BLD-P1
Bleed; ELISA
 After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
03-14-2009
04-15-2009
100
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
04-25-2009
110
BLD-P2
Bleed; ELISA
04-27-2009
112
BLD-F1
Final Bleed
04-29-2009
114
Purify Antibody
 Protein A/G purification of production bleed
Day
68
05-04-2009
119
Shipment
1 ml Pre-bleed from each rabbit

20-50 ml purified rabbit IgGs
ELISA data

1-3 mg of protein

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn