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Your Antibody: Price Antibody Shipping Date Product ID
Mouse hybridomas and purified mouse
monoclonal IgGs for 1 peptide antigen
$3499 As early as 04-23-2009 PE-CA
  No later than 05-07-2009    
1) Peptides conjugated to KLH for immunization and to BSA for ELISA
2) Immunization of 6 Balb/c mice for each peptide-KLH conjugate
3) ELISA monitoring of anti-sera development until titers reach >10,000
4) Fusion of spleen cells from 1 mouse of highest ELISA titer with SP2/0
5) 4-5 rounds of sub-cloning and ELISA screening to establish hybridomas
6) Cryopreservation of cell lines
7) Protein A/G affinity purification of ascites from 2 cell lines

 
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