Order Today To Follow This Production Schedule |
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Receive proteins from customers;
6 mice ready (take pre-bleeds)
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1st Immunization (Complete Freud Adjuvant)
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2nd Immunization (Incomplete Freud Adjuvant)
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3rd Immunization (Incomplete Freud Adjuvant)
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After 3rd Immunization, IF titers>10,000, go on to Fusion and hybridoma cloning: as early as
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4th Immunization (Incomplete Freud Adjuvant)
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03-15-2009 |
69 |
Fusion |
SP2/0 fusion with spleen cells
from 1 mouse (plating in 10X 96-well plates)
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03-25-2009 |
79 |
S1-ELISA
1-Sclone
S2-ELISA 2-Sclone
S3-ELISA 3-Sclone
S4-ELISA 4-Sclone
S5-ELISA |
4-5 rounds of sub-cloning and ELISA
screening
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05-14-2009 |
129 |
05-28-2009 |
143 |
Cryopreservation |
Expand cell lines and make frozen
stocks
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06-18-2009 |
164 |
Ascites
Production/Purification |
Protein A/G purification of ascites
from 2 mice for each cell line
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1) 2 hybridomas (Two 25 cm2 flasks with 2-5 x 106 cells in DMEM/high glucose/15% FBS);
2) 2-5 mg of Protein A/G purified mouse IgGs from ascites production;
3) ELISA data;
4) Cell line isotypes
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