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From Protein Antigens Provided by Customers To 2 Mouse Hybridomas (PE-CL)               

 Production Description
Step 1) Immunization of 6 Balb/c mice for each protein immunogen provided by customers
Step 2) ELISA monitoring of anti-sera development until titers reach >10,000
Step 3) Fusion of spleen cells from 1 mouse of highest ELISA titer with SP2/0
Step 4) 4-5 rounds of sub-cloning and ELISA screening to establish hybridomas
Step 5) Cryopreservation of cloned cell lines

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
11-21-2008
1
Order Date
 
11-28-2008
8
Protein Receive
Receive proteins from customers;
6 mice ready (take pre-bleeds)
11-29-2008
9
Adjuvant Addition
11-30-2008
10
Production Start
11-30-2008
10
IMM-1
1st Immunization (Complete Freud Adjuvant)
12-14-2008
24
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
12-28-2008
38
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
01-07-2009
48
BLD-M1
Bleed; ELISA
After 3rd Immunization, IF titers>10,000, go on to Fusion and hybridoma cloning: as early as
Date
01-13-2009
01-11-2009
52
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
01-21-2009
62
BLD-M2
Bleed; ELISA
Day
53
01-25-2009
66
BST-F2
Final Boost (iv/ip)
01-28-2009
69
Fusion
 SP2/0 fusion with spleen cells from 1 mouse (plating in 10X 96-well plates)
02-07-2009
79
S1-ELISA 1-Sclone
S2-ELISA 2-Sclone
S3-ELISA 3-Sclone
S4-ELISA 4-Sclone
S5-ELISA
4-5 rounds of sub-cloning and ELISA screening
03-29-2009
129
04-12-2009
143
Cryopreservation
Expand cell lines and make frozen stocks
04-17-2009
148
Shipment
1) 2 hybridomas (Two 25 cm2 flasks with 2-5 x 106 cells in DMEM/high glucose/15% FBS);
2) ELISA data;
3) Cell line isotypes

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn