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From Peptide Antigens Provided by Customers To Peptide-affinity purified IgGs (PE-AP)               
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 Production Description
Step 1) Peptides conjugated to KLH for immunization and to BSA for ELISA
Step 2) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate
Step 3) ELISA monitoring of anti-sera development until titers reach >10,000
Step 4) Peptide-affinity purification of production bleed

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
01-06-2009
1
Order Date
  
01-13-2009
8
Peptide Receive
Receive peptides from customers;
Conjugation to KLH (for immunization) and to BSA (for ELISA);
Two rabbits ready (Pre-bleed is done)
01-14-2009
9
Peptide Conjugation
01-15-2009
10
Adjuvant Addition
01-16-2009
11
Production Start
01-16-2009
11
IMM-1
1st Immunization (Complete Freud Adjuvant)
02-13-2009
39
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
02-27-2009
53
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
03-06-2009
60
BLD-P1
Bleed; ELISA After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
03-14-2009
03-13-2009
67
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
03-23-2009
77
BLD-P2
Bleed; ELISA
03-25-2009
79
BLD-F1
Final Bleed
03-28-2009
82
Purify Antibody
Peptide affinity purification of production bleed
Day
68
04-02-2009
87
Shipment

1)1 ml of Pre-bleed from each rabbit
2)1-5 mg of antigen-specific IgGs from each rabbit
3)ELISA data

 

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn