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| From Peptide Antigens Provided by Customers To Anti-sera (PE-AS) |
Production Description |
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| Step 1) Peptides conjugated to KLH for immunization and to BSA for ELISA | |||||
| Step 2) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate | |||||
| Step 3) ELISA monitoring of anti-sera development until titers reach >10,000 | |||||
| Step 4) 30-50 ml of anti-sera from production bleed of each rabbit |
Order Today To Follow This Production Schedule |
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Date |
Day |
AbMART Track |
Production Steps |
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01-06-2009 |
1 |
Order Date |
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01-13-2009 |
8 |
Peptide Receive |
Receive peptides from customers; Conjugation to KLH (for immunization) and to BSA (for ELISA); Two rabbits ready (Pre-bleed is done) |
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01-14-2009 |
9 |
Peptide Conjugation |
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01-15-2009 |
10 |
Adjuvant Addition |
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01-16-2009 |
11 |
Production Start |
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01-16-2009 |
11 |
IMM-1 |
1st Immunization (Complete Freud Adjuvant) | |||
02-13-2009 |
39 |
IMM-2 |
2nd Immunization (Incomplete Freud Adjuvant) | |||
02-27-2009 |
53 |
IMM-3 |
3rd Immunization (Incomplete Freud Adjuvant) | |||
03-06-2009 |
60 |
BLD-P1 |
Bleed; ELISA | After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as | Date |
03-11-2009 |
03-13-2009 |
67 |
IMM-4 |
4th Immunization (Incomplete Freud Adjuvant) | |||
03-23-2009 |
77 |
BLD-P2 |
Bleed; ELISA | |||
03-25-2009 |
79 |
BLD-F1 |
Final Bleed | Day |
65 |
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03-30-2009 |
84 |
Shipment |
1 ml Pre-bleed from each rabbit | |||
| 30-50 ml of anti-sera from each rabbit | ||||||
| ELISA data | ||||||
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