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From Peptide Antigens Provided by Customers To Phospho-specific Peptide-affinity Purified IgGs (PE-AP-M)             
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 Production Description
Step 1) Phospho-peptides conjugated to KLH for immunization and to BSA for ELISA/non-phospho-peptide conjugated to BSA for ELISA
Step 2) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate
Step 3) ELISA monitoring of anti-sera development until titers reach >10,000
Step 4) Double peptide affinity purification of production bleed to yield phospho-specific antibodies

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
11-21-2008
1
Order Date
   
11-28-2008
8
Peptide Receive
 Receive phospho-peptides/control peptides from customers;
Conjugation to KLH (for immunization) and to BSA (for ELISA);
Two rabbits ready (take pre-bleeds)
11-29-2008
9
Peptide Conjugation
11-30-2008
10
Adjuvant Addition
12-01-2008
11
Production Start
12-01-2008
11
IMM-1
 1st Immunization (Complete Freud Adjuvant)
12-29-2008
39
IMM-2
 2nd Immunization (Incomplete Freud Adjuvant)
01-12-2009
53
IMM-3
 3rd Immunization (Incomplete Freud Adjuvant)
01-19-2009
60
BLD-P1
 Bleed; ELISA After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
01-24-2009
01-26-2009
67
IMM-4
 4th Immunization (Incomplete Freud Adjuvant)
02-05-2009
77
BLD-P2
 Bleed; ELISA
02-07-2009
79
BLD-F1
 Final Bleed
02-09-2009
81
Purify Antibody
 Double peptide affinity purification; SDS-PAGE
Day
65
02-14-2009
86
Shipment
 1) 1ml Pre-bleed;
2) 1-5 mg of phospho-specific rabbit IgGs;
3) ELISA data

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn