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From Peptide Antigens Provided by Customers To Phospho-specific Peptide-affinity Purified IgGs (PS-AP-M)             

 Production Description
Step 1) Phospho-peptide and non-phospho-peptide synthesis at AbMART
Step 2) Phospho-peptides conjugated to KLH for immunization and to BSA for ELISA/non-phospho-peptide conjugated to BSA for ELISA
Step 3) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate
Step 4) ELISA monitoring of anti-sera development until titers reach >10,000
Step 5) Double peptide affinity purification of production bleed to yield phospho-specific antibodies

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
01-06-2009
1
Order Date
 
01-26-2009
22
Peptide Synthesis
 Phospho-peptide/non-phospho-peptide synthesis based on sequences provided by customers;
Conjugation of phospho-peptide to KLH (for immunization) and (both peptides) to BSA (for ELISA);
Two rabbits ready (take pre-bleeds)
01-26-2009
25
Peptide Receive
01-27-2009
26
Peptide Conjugation
01-28-2009
27
Adjuvant Addition
01-29-2009
28
Production Start
01-29-2009
28
IMM-1
1st Immunization (Complete Freud Adjuvant)
02-26-2009
56
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
03-12-2009
70
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
03-19-2009
77
BLD-P1
Bleed; ELISA
 After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
03-27-2009
03-26-2009
84
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
04-05-2009
94
BLD-P2
Bleed; ELISA
04-07-2009
96
BLD-F1
Final Bleed
04-13-2009
98
Purify Antibody
Double peptide affinity purification;
SDS-PAGE
Day
82
04-18-2009
103
Shipment
1) 1ml Pre-bleed;
2) 10-20 ml purified rabbit IgGs;
3) ELISA data
4) 1 mg of synthetic peptides

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn