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From Protein Sequences Provided by Customers To Western, IP, IHC and Protein Phosphorylation Western Antibodies
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 Production Description
Step 1) Design and synthesis of 3 peptides at AbMART based on protein sequences provided by customers
Step 2) Peptides conjugated to KLH for immunization and to BSA for ELISA
Step 3) Immunization of 2 New Zealand White rabbits for each peptide-KLH conjugate
Step 4) ELISA monitoring of anti-sera development until titers reach >10,000
Step 5) Peptide affinity purification of production bleeds
Step 6) Generate and optimize antibody data in customer-designated application

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
01-06-2009
1
Order Date
 
01-08-2009
3
Peptide Design
 Peptide synthesis based on sequences provided by customers;
Conjugation to KLH (for immunization) and to BSA (for ELISA);
Two rabbits ready (take pre-bleeds)
01-26-2009
21
Peptide Synthesis
01-25-2009
20
Peptide Receive
01-26-2009
21
Peptide Conjugation
01-27-2009
22
Adjuvant Addition
01-28-2009
23
Production Start
01-28-2009
23
IMM-1
1st Immunization (Complete Freud Adjuvant)
02-25-2009
51
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
03-11-2009
65
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
03-18-2009
72
BLD-P1
Bleed; ELISA
 After 3rd Immunization, IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
03-14-2009
03-25-2009
79
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
04-04-2009
89
BLD-P2
Bleed; ELISA
04-06-2009
91
BLD-F1
Final Bleed
04-08-2009
93
Purity Antibody
 Peptide affinity purification;
SDS-PAGE
Day
68
04-23-2009
107
Application Data
 Generate and optimize Western/IP/IHC/ Phosphorylation data
04-27-2009
112
Shipment
 1 ml Pre-bleed;
1-5 mg of antigen-specific IgGs from each rabbit;
Application data package;
1 mg of synthetic peptide

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn