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From Protein Antigens Provided by Customers To Protein-affinity Purified IgGs (PR-AP)               

 Production Description
Step 1) Immunization of 2 New Zealand White rabbits for each protein immunogen
Step 2) ELISA monitoring of anti-sera development until titers reach >10,00
Step 3) Protein-affinity purification of production bleed

 Order Today To Follow This Production Schedule
Date
Day
AbMART Track
Production Steps
01-06-2009
1
Order Date
 
01-13-2009
8
Protein Receive
 Receive proteins from customers;
Two rabbits ready (take pre-bleeds)
01-14-2009
9
Adjuvant Addition
01-15-2009
10
Production Start
01-15-2009
10
IMM-1
1st Immunization (Complete Freud Adjuvant)
02-12-2009
38
IMM-2
2nd Immunization (Incomplete Freud Adjuvant)
02-26-2009
52
IMM-3
3rd Immunization (Incomplete Freud Adjuvant)
03-05-2009
59
BLD-P1
Bleed; ELISA
 IF titers>10,000, go on to Final Bleed: Shipment as early as
Date
03-12-2009
03-12-2009
66
IMM-4
4th Immunization (Incomplete Freud Adjuvant)
03-22-2009
76
BLD-P2
Bleed; ELISA
03-24-2009
78
BLD-F1
Final Bleed
03-27-2009
81
Purify Antibody
Protein affinity purification of production bleed
Day
67
04-01-2009
86
Shipment

1 ml Pre-bleed from each rabbit
1-5 mg of antigen-specific IgGs from each rabbit
ELISA data

2140 Xietu Road, Building 2 Shanghai, P.R. China 200032, Phone: (011)-86-21-64048593, Email: orders@ab-mart.com.cn