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No.
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Size
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Price
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P30010
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100 μl
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￥900
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Product Name
GFP Antibody
Source
This Abmart polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of EGFP.
Specifity
GFP Antibody detects GFP and GFP fusion proteins.
Storage
Store at -20°C. Stable for one year from the date of shipment.
Secondary Antibody (Important)
Use an anti-RABBIT secondary antibody to detect the GFP Antibody.
Application Tested
Western blotting 1:2000 Immunoprecipitation 1:100
Application Data
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Western blot analysis of over-expressed GFP protein in COS7 cell lysate, using GFP rabbit polyclonal antibody. The antibody dilutions are 1:1000 (lane1), 1:2000 (lane2), 1:5000 (lane3), 1:10000 (lane4). Each lane was loaded with 10 µg of cell lysate.
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IP of extracts from COS7 cells transfected with a GFP-tagged protein using GFP rabbit polyclonal antibody (5 µl, lane 3) or using normal rabbit IgG (lane 2) as control, followed by Western blot using Abmart GFP (7G9) Mouse mAb (dilution: 1:5000). Lane 1 was loaded with 10 µg of COS7 cell lysate over-expressing a GFP-tagged protein.
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AbMATE-2A8 A positive control for using
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Failure to see above result suggests that your Western experiment system is NOT
working, and likely your own Western experiment will not work with
. If you see the expected band and the dot (
is working), but your own experiment membrane is not what you expected, please look
for other reasons (for example, your protein sample preparation) rather than Western
system itself.
Please contact Abmart’s technical support team by phone (+86-21-64048322) or email
(support@abmart.com.cn) for further questions.
AbMATE-
can be stored at 4°C for 6 months.
(The AbMATE-
should be submerged in methanol for 5 minutes before using).
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Membrane before experiment
Film after experiment
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Companion Products
For in vitro research use only and not intended for use in humans or animals