Loading Control Antibodies

No. Size Price
M20028S 50ul ￥500
M20028M 100ul ￥900
M20028L 200ul ￥1600

    download Datasheet PDF

Product Name

GAPDH(2A8) mAb

Description

Isotype

Mouse IgG1

Molecular Weight

37 kDa

Source

This Abmart monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) of human GAPDH.

Specifity

GAPDH (2A8) Mouse mAb detects endogenous levels of total GAPDH protein.

Aliases

Storage

Store at -20°C. Stable for one year from the date of shipment.

Secondary Antibody (Important)

Use an anti-MOUSE secondary antibody.

Application Tested

WB 1: 2000-1:5000

Reactivity

H, R, M, Mk, Dg, ChHm

Application Data

WB WB

Western blot analysis of extracts from various cell lines:
Lane 1-8: MCF-7, A549, HepG2, BHK, C2C12, mouse skeletal muscle, mouse heart muscle, mouse brain.
Primary antibody: 1: 5000

Western blot analysis of extracts from various cell lines:
Lane 1-8: Hela, HEK293, Cos-7, NIH/3T3, CHO-K1, MDCK, P12, C6
Primary antibody: 1: 2000

IF

IF

WB WB

IHC IHC

Applications Key

Reactivity Key

AbMATE-2A8  A positive control for using

  • 1)
  • 2)

Failure to see above result suggests that your Western experiment system is NOT working, and likely your own Western experiment will not work with .  If you see the expected band and the dot ( is working), but your own experiment membrane is not what you expected, please look for other reasons (for example, your protein sample preparation) rather than Western system itself.

Please contact Abmart’s technical support team by phone (+86-21-64048322) or email (support@abmart.com.cn) for further questions.

AbMATE- can be stored at 4°C for 6 months.

(The AbMATE- should be submerged in methanol for 5 minutes before using).

Mate
Membrane before experiment

Mate
Film after experiment

Companion Products

For in vitro research use only and not intended for use in humans or animals