Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation. The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome.

Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) (Kelch-like protein 19) KEAP1 INRF2 KIAA0132 KLHL19

Human, Mouse, Rat, Monkey

Pig,Bovine,Horse,Sheep,Rabbit,Dog

WB,IHC,IF/ICC,ELISA

WB,IHC,IF/ICC, ELISA Recommended dilution WB 1:500-1:2000 IHC 1:50-1:500 IF/ICC 1:50-1:500 ELISA 1：2000

70kD; 70kDa(Calculated).

KEAP1 INRF2 KIAA0132 KLHL19

Q14145

https://www.uniprot.org/uniprotkb/q14145

Rabbit

Polyclonal

Rabbit IgG

Keap1 Antibody detects endogenous levels of total Keap1.

PBS,pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Store at -20 °C. Stable for 12 months from date of receipt.

AB_2936804