Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate . Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines . In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) . Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis . May play a role in sperm motility .

P00558

5230

Phosphoglycerate kinase 1, 2.7.2.3, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA

Human,Mouse

WB,IHC,FC

WB 1:8000
IHC 1:100-500
FC 1:25

45 kD

Mouse

monoclonal

IgG2a,k

This antibody is purified through a proteinG column.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.