Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5\\'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5\\'-ssDNA, while the 3\\'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5\\'-3\\' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5\\'-3\\' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

DNA replication ATP-dependent helicase/nuclease DNA2 (hDNA2) (DNA replication ATP-dependent helicase-like homolog) [Includes: DNA replication nuclease DNA2 (EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2 (EC 3.6.4.12)] DNA2 DNA2L KIAA0083

Human

IHC,ELISA

IHC 1:20-1:200
ELISA 1:5000-1:20000

120.415 kDa ;

DNA2 DNA2L KIAA0083

p51530

https://www.uniprot.org/uniprotkb/p51530

Recombinant Human DNA replication ATP-dependent helicase/nuclease DNA2 protein (1-300AA)

Rabbit

Polyclonal

IgG

Epigenetics and Nuclear Signaling

>95%, Protein G purified

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Store at -20°C. Do not aliquot the antibody