Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5\\\\\\'- and 3\\\\\\'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5\\\\\\'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5\\\\\\'-RGAAGAAC-3\\\\\\' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5\\\\\\'-CGAGGCG-3\\\\\\' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

Serine/arginine-rich splicing factor 1 (Alternative-splicing factor 1) (ASF-1) (Splicing factor, arginine/serine-rich 1) (pre-mRNA-splicing factor SF2, P33 subunit) SRSF1 ASF SF2 SF2P33 SFRS1 OK/SW-cl.3

Human

IHC,ELISA

IHC 1:20-1:200
ELISA 1:5000-1:20000

27.745 kDa ;

SRSF1 ASF SF2 SF2P33 SFRS1 OK/SW-cl.3

q07955

https://www.uniprot.org/uniprotkb/q07955

Recombinant Human Serine/arginine-rich splicing factor 1 protein (1-248AA)

Rabbit

Polyclonal

IgG

Epigenetics and Nuclear Signaling

>95%, Antigen Affinity Purified

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Store at -20°C. Do not aliquot the antibody