Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1) AAK1 KIAA1048

Human, Mouse

WB,IHC,IF,ICC,ELISA

WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
ICC 1:100-1:400
ELISA 1:5000-1:20000

103.885 kDa ;

AAK1 KIAA1048

q2m2i8

https://www.uniprot.org/uniprotkb/q2m2i8

Recombinant Human AP2-associated protein kinase 1 protein (274-465AA)

Rabbit

Polyclonal

IgG

Transmembrane

Signal transduction

>95%, Protein G purified

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Store at -20°C. Do not aliquot the antibody