13162017139
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
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Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand . Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion . .
Proliferating cell nuclear antigen (PCNA) (Cyclin) PCNA
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
29kD
PCNA
P12004,P17918,P04961
Rabbit
Polyclonal
Rabbit IgG
Product Information
| Description | PCNA rabbit polyclonal |
| Protein full name | Proliferating cell nuclear antigen |
| Synonyms | PCNA, ATLD2, proliferating cell nuclear antigen |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW. | 33 kDa |
| Observed MW. | 33 kDa |
| Subcellular location | Nucleus |
| Uniprot ID | P12004,P17918,P04961 |
Applications
| Applications | WB | IHC | IF |
| Species | Human,Mouse,Rat | Human,Mouse,Rat | Human,Mouse,Rat |
| Dilution | 1:2000 | 1:300-1:100 | 1:200-1:500 |
| Positive tissue | Hela,jurkat,Raw264.7 | liver,lung,spleen | Hela,lung |
Background
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Images
| Western blot analysis of PCNA (T61010M) at dilution of 1:2000. Lane 1: hela cell lysate Lane 2: Jurkat cell lysate Lane 3: Raw264.7 cell lysate Lane 4: NRK-52E cell lysate Lane 5: Human heps tissue lysate |
 | Immunohistochemistry analysis of paraffin-embedded human liver cancer using PCNA (T61010M) at dilution of 1:500. |
 | Immunohistochemistry analysis of paraffin-embedded mouse lung using PCNA (T61010M) at dilution of 1:500. |
| Immunohistochemistry analysis of paraffin-embedded rat spleen using PCNA (T61010M) at dilution of 1:500. |
 | Immunofluorescence analysis of paraffin-embedded mice hela tumor using PCNA (T61010M) at dilution of 1:500. |
 | Immunofluorescence analysis of paraffin-embedded mouse lung using PCNA (T61010M) at dilution of 1:200. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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应聘职位：hr@ab-mart.com
订购专线：4006-123-828
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程经理：手机18616261485（微信同号）
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