LPS-PG is a standard preparation of lipopolysaccharide (LPS) from the Gram-negative bacteria Porphyromonas gingivalis. LPS is the principal component of Gram negative bacteria that activates the innate immune system. LPS recognition is predominantly mediated by TLR41. LPS-PG is an important virulence factor in the mechanisms of peridontal disease. LPS-PG presents a unique and heterogenous chemical structure, which differs from traditionally recognized enteric bacterium-derived LPS. The fact that LPS-PG exhibits activity in C3H/HeJ mice, which are deficient for TLR4, led to a common belief that this LPS is a TLR2 ligand2,3. However, structural and functional studies of LPS-PG have revealed that it activates cells through TLR4. The TLR2 activity of LPS-PG is ascribed to a contaminant lipoprotein4. The TLR response to LPS-PG is dependent on the presence of key accessory molecules: CD14 is required for both TLR2 and TLR4 activation while MD-2 is

only necessary for TLR4 activation5.

1. Poltorak A. et al., 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science, 282:2085-8.

2. Kirikae T. et al., 1999. Lipopolysaccharides (LPS) of oral black-pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a way different from that of Salmonella LPS. Infect Immun. 67(4):1736-42.

3. Hirschfeld M. et al., 2001. Signaling by toll-like receptor 2 and 4 agonists results in differential gene expression in murine macrophages. Infect Immun. 69(3):1477-82.

4. Ogawa T. et al., 2007. Chemical structure and immunobiological activity of Porphyromonas gingivalis lipid A. Front Biosci. 12:3795-812.

5. Darveau RP. et al., 2004. Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both toll-like receptors 2 and 4. Infect Immun. 72(9):5041-51.

LPS-PG is provided lyophilized and shipped at room temperature. Store at -20°C.

Upon resuspension, prepare aliquots of LPS-PG and store at 4°C for short term storage or -20°C for long storage.

Resuspended product is stable for 1 month at 4°C and for 6 months at -20°C when properly stored.

The TLR4 activity is controlled using HEK-Blue? TLR4 cells.

The presence of other bacterial components (e.g. lipoproteins) is controlled using HEK-Blue? TLR2 cells.

Preparation of stock solution (1 mg/ml)

- Add 1 ml of endotoxin-free water (provided) and homogenize.

- Prepare aliquots of stock solution and store at 4°C for 1 month or at -20°C for 6 months. Further dilutions can be prepared using water.

? TLR4 activity: 100 ng - 10 μg/ml

? TLR2 activity: 10 ng/ml - 10 mg/ml

特定的存储和包装每个产品的信息在产品说明书上都有注明。大多数产品，在推荐的条件下存储可稳定保存两年。产品有时建议的储存温度不同，大多数建议储存在-20°C，抑制剂属于化学试剂，可在常温下运输储存两周左右。即使如此，我们保证产品的出货量将保持产品质量的条件下，一般都会放入冰袋。望阁下收到产品后，请按照产品数据表建议适当存储。

特定的存储和包装每个产品的信息在产品说明书上都有注明。大多数产品，在推荐的条件下存储可稳定保存两年。产品有时建议的储存温度不同，大多数建议储存在-20°C，抑制剂属于化学试剂，可在常温下运输储存两周左右。即使如此，我们保证产品的出货量将保持产品质量的条件下，一般都会放入冰袋。望阁下收到产品后，请按照产品数据表建议适当存储。

#M20001 His-Tag (2A8) Mouse mAb
#M20002 Myc-Tag (19C2) Mouse mAb
#M20003 HA-Tag (26D11) Mouse mAb
#M20004 GFP-Tag (7G9) Mouse mAb
#M20007 GST-Tag (12G8) Mouse mAb
#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma"s Anti-FLAG M2 Antibody)
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)

#M20018 Anti-DYKDDDDK-Tag Mouse Antibody (Agarose Conjugated) (Same as Sigma"s Anti-FLAG M2)

#M20118 Anti-DYKDDDDK-Tag Mouse Antibody (Magnetic Beads) (Same as Sigma"s Anti-FLAG M2)