Histones are basic nuclear proteins that are responsible for the nucleosome structure of  the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of  DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four  core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through  the interaction of a linker histone, H1, with the DNA between the nucleosomes to form  higher order chromatin structures. This gene is intronless and encodes a replication?dependent histone that is a member of the histone H4 family. Transcripts from this gene  lack polyA tails; instead, they contain a palindromic termination element. This gene is  found in a histone cluster on chromosome 1. This gene is one of four histone genes in the  cluster that are duplicated; this record represents the centromeric copy.

FO108;H4;H4/n;H4F2;H4FN;HIST2H4;Histone H4;HIST1H4A;HIST2H4A

A synthetic peptide of human Acetyl-Histone H4-K8.

Rabbit lgG

Human, Mouse, Rat, Other (Wide Range)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone  H4-K8 antibody at1:1000 dilution.NIH/3T3 cells and C6 cells were treated  by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane.

Western blotting   1:500-1:2000  

IF/IHC/IP   1:50-1:200 

ChIP   1:20-1:100

Store at -20℃ Stable far one year from the date of shipment.