Source: Prokaryotic expression.

Host: E. coli

Residues: Ile28~Lys257

Tags: N-terminal His-tag

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Buffer Formulation: PBS, pH7.4, containing 0.01% Sarcosyl, 5% Trehalose.

Original Concentration: 350μg/mL

Applications: Activity Assays.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.5

Predicted Molecular Mass: 29.6kDa

Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8^oC for one month.

             Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

AMPK alpha 1, also known as AMP-activated protein kinase alpha 1 subunit, is a crucial enzyme that plays a significant role in energy metabolism. It is is involved in regulating several metabolic processes, including glucose and fatty acid uptake, oxidation, and storage. It promotes energy-producing pathways, such as glycolysis, fatty acid oxidation, and mitochondrial biogenesis, while inhibiting energy-consuming processes, like protein synthesis and lipogenesis. Besides, PRKAb1 has been identified as an interactor of AMPK Alpha 1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human AMPK Alpha 1 and recombinant human PRKAb1. Briefly, AMPK Alpha 1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PRKAb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AMPK Alpha 1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human AMPK Alpha 1 and recombinant human PRKAb1 was shown in Figure 1, the EC50 for this effect is 0.94 ug/mL.

Figure 1. The binding activity of recombinant human AMPK Alpha 1 and recombinant human PRKAb1

Figure 2. Gene Sequencing (extract)

Figure 3. SDS-PAGE

Sample: Active recombinant AMPK Alpha 1, Human

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.