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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
The Wnt genes are a group of conserved, Cysteine-rich, secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the transmembrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation. Reduced levels of active GSK causes accumulation of b-catenin and subsequent regulation of developmentally significant Wnt target genes. Wnt antagonists such as dickkopf (Dkk), frizzled-related protein (sFRP) and Wnt inhibitory factor-1 (WIF-1), are necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes. WIF-1 is a 379-amino acid secreted protein that contains an N-terminal signal sequence, a 150-amino acid WIF domain, five epidermal growth factor-like repeats and a 45-amino acid C-terminal hydrophilic domain.
Wnt inhibitory factor 1 (WIF-1) WIF1 UNQ191/PRO217
Human,Mouse
WB,IHC,IF,ICC
WB 1:500IHC 1:50-1:200IF 1:100-1:400ICC 1:50-1:200
42 kDa
WIF1 UNQ191/PRO217
swissprot: q9y5w5 human;swissprot: q9wua1 mouse
Mouse
Monoclonal
Mouse IgG1
Stem cells->Signaling Pathways->Wnt->Secreted
Hela, NIH/3T3, NTERA-2, human ovary tumour tissue, human lung cancer tissue.
Protein A affinity purified.
Liquid
2 mg/mL.
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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