Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a complex that mediates the vacuolar degradative transport via the intracellular vesicle trafficking from the endosome to the vacuole. Probably regulates the PIN1 and PIN2 homeostasis through its interaction with SNX1. An eight-subunit protein complex, BLOC-1, which is involved in endosomal transport from the endosomes to the lysosomes, has been identified in both human and mice. In this study, two homologous subunits of this complex, BLOS1 (or AtGCN5L1) and BLOS2, have been characterized in Arabidopsis. Both BLOS1 and BLOS2 interacted with SNX1 on the sorting endosomes. Inducible RNAi lines with reduced levels of BLOS1 had longer primary roots and more lateral roots. Consistently, PIN1 and PIN2 were increased in BLOS1 RNAi lines, implicating an impaired transport from the endosomes to the vacuoles. These results suggest that a putative BLOC-1 complex in Arabidopsis might mediate the vacuolar degradative transport through direct interaction with SNX1 to regulate the homeostasis of PIN1 and PIN2, which is important for plant growth and development.
Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC-1 subunit 1) (GCN5-like protein 1) (Protein RT14 homolog) BLOS1 GCN5L1 At2g30330 T09D09.14
Rice,Arabidopsis thaliana
WB,IHC
WB 1:500-1:2000IHC 1:50-1:200
17 kDa
BLOS1 GCN5L1 At2g30330 T09D09.14
swissprot: o22929 arath
Rabbit
Polyclonal
Rabbit IgG
Rice tissue lysates, A. thaliana tissue.
Peptide affinity purified.
Liquid
1 mg/mL
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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