PU661033

货号 规格 价格(¥)
PU661033S 50μl
PU661033M 100μl

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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经典一抗-NLK antibody

Datasheet

Background

Nlk Antibody (B-5) is a high quality monoclonal Nlk antibody (also designated Nlk antibody) suitable for the detection of the Nlk protein of mouse, rat and human origin. Nlk Antibody (B-5) is available as both the non-conjugated anti-Nlk antibody form, as well as multiple conjugated forms of anti-Nlk antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2 (1-4), respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both Thr 183 and Tyr 185 is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene. Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases. Nlk is a nuclear protein with the ability to autophosphorylate.

Alternative Name

Serine/threonine-protein kinase NLK (EC 2.7.11.24) (Nemo-like kinase) (Protein LAK1) NLK LAK1

Reactivity

Human,Mouse

Predicted Reactivity

Application

WB,IHC,IF,ICC

Application Image

Recommended Dilution

WB 1:500-1:2000
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:50-1:100

Mol Weight

58 kDa

Gene Symbol

NLK LAK1

Uniprot

swissprot: q9ube8 human;swissprot: o54949 mouse

Uniprot link

Immunogen

Immunogen Range

Source

Rabbit

Clonality

Polyclonal

Isotype

Rabbit IgG

Specificity

Subcellular location

Research Area

Stem cells->Signaling Pathways->Wnt->Nuclear

Positive control

SW480 cell lysate, HCT116 cell lysate, C2C12 cell lysate, MG-63, SW620, human uterus tissue, human small intestine tissue, human breast carcinoma tissue.

Conjugation

Modification

Purification

Protein affinity purified.

Formulation

Liquid

Concentration

1 mg/mL.

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

产品介绍

Clone

Target

波长

Storage

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.

注意事项

Companion Products

Important

Comments

RRID

客户评价

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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