Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Glycerol kinase, encoded by the gene GK, is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol 3-phosphate. Adipocytes lack glycerol kinase so they cannot metabolize the glycerol produced during triacyl glycerol degradation. This glycerol is instead shuttled to the liver via the blood where it is: Phosphorylated by glycerol kinase to glycerol 3-phosphate. Converted from glycerol 3-phosphate to dihydroxyacetone phosphate (DHAP) via glycerol 3-phosphate dehydrogenase. DHAP can participate in glycolysis or gluconeogenesis. This protein may use the morpheein model of allosteric regulation. Glycerol Kinase (alternative name, ATP:glycerol 3-phosphotransferase or Glycerokinase) adopts a ribonuclease H-like fold consisting of an alpha-beta 2-layer sandwich of CATH family 3.30.420.40. As of March 2010, there were 20 structures of this protein in the PDB, most of which are homodimeric.
Glycerol kinase (Glycerokinase) (EC 2.7.1.30) (ATP:glycerol 3-phosphotransferase) GK
Human,Mouse,Rat
WB,IHC,FC
WB 1:500-1:2000IHC 1:50-1:200FC 1:50-1:100
Predicted band size 61/57 kDa.
GK
swissprot: p32189 human;swissprot: q64516 mouse;swissprot: q63060 rat
Rabbit
Monoclonal
Rabbit IgG
Cancer->Cancer Metabolism->Metabolic signaling pathway->Metabolism of lipids and lipoproteins;Metabolism->Pathways and Processes->Metabolic signaling pathways->Energy transfer pathways->Energy Metabolism;Metabolism->Pathways and Processes->Metabolic signaling pathways->Lipid and lipoprotein metabolism->Lipid metabolism
Mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse bone marrow tissue lysate, rat testis tissue lysate, rat testis tissue, human liver tissue, human kidney tissue, mouse testis tissue, 293.
Protein A affinity purified.
Liquid
1 mg/mL.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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