Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The Human,Pig caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] CASP3 CPP32

Human,Pig

WB,IHC,IF,ICC

WB 1:1000-1:2000
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:50-1:200

17 kDa

CASP3 CPP32

swissprot: p42574 human,pig

https://www.uniprot.org/uniprotkb/p42574

Rabbit

Monoclonal

Rabbit IgG

Neuroscience->Neurology process->Neurogenesis;Neuroscience->Neurology process->Neurodegenerative disease->Alzheimer's disease->Other;Cell Biology->Proteolysis / Ubiquitin->Proteolytic enzymes->Other proteases;Cell Biology->Apoptosis->Intracellular->Caspases etc(341)->Caspases;Cancer->Invasion/microenvironment->Apoptosis->Death receptors ligands->TRAF;Cancer->Invasion/microenvironment->Apoptosis->Caspases;Cancer->Invasion/microenvironment->Apoptosis->Chromatin condensation;Cancer->Invasion/microenvironment->Angiogenesis->Angiogenic growth factors;Cancer->Invasion/microenvironment->Angiogenesis->Angiogenic inhibitory factors;Cancer->Invasion/microenvironment->Angiogenesis->Other;Cancer->Invasion/microenvironment->Hypoxia->Hypoxia-related proteins;Cancer->Tumor biomarkers->Tumor antigens;Cancer->Cell Death->Apoptosis->Apoptosis Markers->Caspases

Camptothecin (2 μM) treated Jurkat cell lysate, Hela, PC-3M, Human,Pig tonsil tissue, Human,Pig spleen tissue.

Protein A affinity purified.

Liquid

1 mg/mL.

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.