Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
Proliferating cell nuclear antigen (PCNA) (Cyclin) PCNA
Human,Mouse,Rat
WB,IHC,IF,ICC,IP,FC
WB 1:5000-1:20000IHC 1:1000-1:10000IF 1:200-1:500ICC 1:1000-1:2000IP 1:50FC 1:200
29 kDa
PCNA
swissprot: p12004 human;swissprot: p17918 mouse;swissprot: p04961 rat
Rabbit
Monoclonal
Rabbit IgG
Nucleus localization
Cell Biology->Cell Cycle->Markers;Cancer->Cell cycle->Cell division
Hela cell lysate, 293 cell lysate, A431 cell lysate, human tonsil tissue, human spleen tissue, human breast carcinoma tissue, mouse colon tissue, mouse spleen tissue, mouse stomach tissue, Hela.
Protein A affinity purified.
Liquid
1 mg/mL.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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订购专线:4006-123-828
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