Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-) GAPDH GAPD CDABP0047 OK/SW-cl.12

Human,Mouse,Rat,Chicken,Drosophila melanogaster,Zebrafish

WB,IHC,IF,ICC,IP,FC

WB 1:50000-1:200000
IHC 1:1000
IF 1:200-1:500
ICC 1:1000
IP 1-2μg/sample
FC 1:1000

36 kDa

GAPDH GAPD CDABP0047 OK/SW-cl.12

SwissProt: P04406 Human;SwissProt: P16858 Mouse;SwissProt: P04797 Rat

https://www.uniprot.org/uniprotkb/p04406

Rabbit

Monoclonal

Rabbit IgG

Neuroscience->Neurology process->Neurodegenerative disease->Huntington's disease;Neuroscience->Neurology process->Neurodegenerative disease->Alzheimer's disease->Other;Cancer->Cancer Metabolism->Metabolic signaling pathway->Metabolism of carbohydrates;Metabolism->Pathways and Processes->Metabolic signaling pathways->Energy transfer pathways->Energy Metabolism;Metabolism->Types of disease->Neurodegenerative disease

Rat liver tissue lysate, rat lung tissue lysate, rat heart tissue lysate, rat cerebellum tissue lysate, rat skeletal muscle tissue lysate, rat spleen tissue lysate, rat small intestine tissue lysate, hybrid fish (crucian-carp) brain tissue lysates, PC-3 cell lysate, mouse colon tissue lysate, SHSY-5Y cell lysate, NIH/3T3 cell lysate, SKBR3 cell lysate, rat brain tissue lysate, A549, HepG2, human liver tissue, mouse liver tissue, mouse spleen tissue.

Protein A affinity purified.

Liquid

1 mg/mL.

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.