Anti-NT3 (Neurotrophin 3) monoclonal antibody

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MG360115

货号规格价格(￥)

MG360115S 50ul

MG360115M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Anti-NT3 (Neurotrophin 3) monoclonal antibody

Datasheet

Background

NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. It is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse

Alternative Name

Neurotrophin-3 (NT-3) (HDNF) (Nerve growth factor 2) (NGF-2) (Neurotrophic factor) NTF3

Reactivity

Human, Mouse, Rat

Predicted Reactivity

Application

WB,IHC

Application Image

Recommended Dilution

WB 1:5000
IHC 1:50

Mol Weight

27.4 kDa

Gene Symbol

NTF3

Uniprot

p20783

Uniprot link

https://www.uniprot.org/uniprotkb/p20783

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 139-257 of human NT3

Immunogen Range

Source

Mouse

Clonality

Monoclonal

Isotype

Mouse IgG

Specificity

Subcellular location

Research Area

Neurogenesis

Positive control

Conjugation

Modification

Purification

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Formulation

Liquid

Concentration

0.2 mg/ml

Buffer

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

产品介绍

Clone

Target

波长

Storage

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.

注意事项

Companion Products

Important

Comments

RRID

客户评价

Figure from citation: Detection of NT-3 (NTF3) protein in brain metastases from breast cancer patients. (a) × 100 magnification of the brain metastasis tissue section with the isotype-matched negative control antibody staining. (b) ×100 magnification of the brain metastasis tissue section with the NT-3 antibody staining. View Citation

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-NTF3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF3.