Western blot analysis of extracts(10ug) from a mouse cell line and 3 different mouse tissues by using anti-PRKAR1A monoclonal antibody(1:200).

Equivalent amounts of cell lysates(10 ug per lane) of wild-type 293T cells and PRKAR1A-Knockout 293T cells(KO) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A monoclonal antibody MG730641,(1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.

Western blot analysis of extracts(35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.

HEK293T cells were transfected with the pCMV6-ENTRY control(Left lane) or pCMV6-ENTRY PRKAR1A(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates(5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A(Cat# MG730641). Positive lysates can be purchased separately from Abmart.

Anti-PRKAR1A mouse monoclonal antibody(MG730641) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1A .

Immunohistochemical staining of paraffin-embedded normal breast using anti-PRKAR1A C/N MG730641 clone OTI6C7 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min,anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded breast cancer using anti-PRKAR1A C/N MG730641 clone OTI6C7 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min,anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG730641,Dilution 1:50)

Immunohistochemical staining of paraffin-embedded renal cancer using anti-PRKAR1A C/N MG730641 clone OTI6C7 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min,anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded colon cancer using anti-PRKAR1A C/N MG730641 clone OTI6C7 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min,anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG730641,Dilution 1:50)

Immunohistochemical staining of paraffin-embedded normal colon using anti-PRKAR1A C/N MG730641 clone OTI6C7 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min,anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded melanoma using anti-PRKAR1A C/N MG730641 clone OTI6C7 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min,anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG730641,Dilution 1:50)

Figure from citation: PRKAR1A IHC in a CNC-associated cardiac myxoma. A,A photomicrograph of the specimen at low power exhibits the robust reactivity of the adjacent normal myocardium with antibodies directed against PRKAR1A. B,A high-power photomicrograph exhibits the lack of reactivity seen in the neoplastic(myxoma) cells,although the intratumoral histiocytes are still strongly reactive. Dilution: 1:8000

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG730641,Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG730641,Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG730641,Dilution 1:50)