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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin (By similarity). Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter. .
Collagen alpha-1(V) chain Col5a1
Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
184kD
Col5a1
O88207,Q9JI03
Rabbit
Polyclonal
Rabbit IgG
Product Information
| Description | Collagen Vrabbit polyclonal |
| Protein full name | Collagen alpha-1(V) chain |
Synonyms | Col5a1, Collagen Type V, Alpha 1 type V collagen, Col5A2, Col5A3, Collagen alpha 2(V) chain, Collagen type V alpha 1 |
| Immunogen | KLH conjugated Synthetic peptidecorresponding to MouseCollagen V |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Extracellular region or secreted |
Predicted MW. | 184kDa |
Observed MW. | 184kDa |
| Uniprot ID |
Applications
| Applications | WB | IHC | IF |
| Species | Mouse,Rat | Mouse,Rat | Mouse,Rat |
| Dilution | 1:500-1:1000 | 1:1500-1:3000 | 1:750-1:1500 |
| Positive tissue | liver fibrosis | skin, heart | skin, heart |
Background
COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Images
 | Western blot analysis of Collagen V(T611012M) at dilution of 1: 1000 Lane 1: Mouse liver fibrosis tissue lysate |
 | Immunohistochemistry analysis of paraffin-embedded mouse skin usingCollagen V (T611012M) at dilution of 1: 1800 |
 | Immunohistochemistry analysis of paraffin-embedded mouse heart usingCollagen V (T611012M) at dilutionof 1: 1800 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
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程经理：手机18616261485（微信同号）
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徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
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