13916964679
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. .
Securin (Esp1-associated protein) (Pituitary tumor-transforming gene 1 protein) (Tumor-transforming protein 1) (hPTTG) PTTG1 EAP1 PTTG TUTR1
Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
23kD
PTTG1 EAP1 PTTG TUTR1
O95997,Q9CQJ7,P97613
Rabbit
Polyclonal
Rabbit IgG
Cell division #DNA excision #Spermatogenesis
Product Information
| Description | Securinrabbitpolyclonal |
| Protein full name | Securin |
Synonyms | Pituitary tumor-transforming gene 1 protein,Pttg1, Pttg,Cut2,EAP1, ESP1,TUTR1 |
| Immunogen | KLH conjugated Synthetic peptidecorresponding to MouseSecurin |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Nucleus,Cytoplasm |
Predicted MW. | 22kDa |
Observed MW. | 26kDa |
| Uniprot ID |
Applications
| Applications | WB | IHC | IF |
| Species | Mouse,Rat | Human,Mouse,Rat | Human,Mouse,Rat |
| Dilution | 1:500-1:1000 | 1:1000-1:2000 | 1:500-1:1000 |
| Positive tissue | testis, cecum, skin, epityphlon | tonsil, placenta, testis | tonsil, placenta, testis |
Background
PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The protein has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. This protein contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the protein can act as a transactivation domain. It is mainly a cytosolic protein, although it partially localizes in the nucleus.
Images
| Western blot analysis of Securin (T611047M)at dilution of 1: 800 Lane 1: Mouse testis tissue lysate Lane 2: Mouse cecum tissue lysate Lane 3: Rat testis tissue lysate Lane 4: Rat skin tissue lysate Lane 5: Rat epityphlon tissue lysate |
 | Immunohistochemistry analysis of paraffin-embeddedhuman tonsilusingSecurin (T611047M)at dilution of 1:1400 |
| Immunohistochemistry analysis of paraffin-embedded mouseplacentausingSecurin (T611047M)at dilution of 1:1400 |
| Immunohistochemistryanalysis of paraffin-embeddedrat testisusingSecurin (T611047M)at dilution of 1:1400 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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应聘职位：hr@ab-mart.com
订购专线：4006-123-828
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程经理：手机18616261485（微信同号）
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徐经理：手机15618191473（微信同号）
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