13916964679
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
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Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 . In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators . Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha . Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation . In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity). .
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF) IL10
Rat,Mouse
IHC,IF,ICC
IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
21kD
IL10
P22301,P29456
Rabbit
Polyclonal
Rabbit IgG
defense response to bacterium
Product Information
Description | Il-10 rabbit polyclonal |
Protein full name | Interleukin-10 |
Synonyms | IL10, CSIF, GVHDS, IL-10, IL10A, TGIF, interleukin 10 |
Immunogen | KLH conjugated Synthetic peptide corresponding to MouseIL-10 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | |
Uniprot ID | P22301,P29456 |
Applications
applications | species | dilution | Positive tissue |
IHC | Mouse,Rat | 1:200-1:1000 | spleen,lung |
IF | Mouse,Rat | 1:200-1:1000 | spleen,ung |
Background
IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
Images
| Immunohistochemistry analysis of paraffin-embedded mouse spleen using IL-10 (T61108M) at dilution of 1:300. |
| Immunohistochemistry analysis of paraffin-embedded rat lung using IL-10 (T61108M) at dilution of 1:300. |
| Immunohistochemistry analysis of paraffin-embedded rat spleen using IL-10 (T61108M) at dilution of 1:300. |
| Immunofluorescence analysis of paraffin-embedded mouse Inflammatory lung using IL-10 (T61108M) at dilution of 1:300. |
| Immunofluorescence analysis of paraffin-embedded rat Inflammatory lung using IL-10 (T61108M) at dilution of 1:600. |
| Immunofluorescence analysis of paraffin-embedded rat Inflammatory spleen using IL-10 (T61108M) at dilution of 1:600. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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张经理：手机13122150513（微信同号）
微信客服
邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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