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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes . Contributes to the regulation of filopodia extension . Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan . Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity). .
Ras-related protein Rab-5A (EC 3.6.5.2) RAB5A RAB5
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
24kD
RAB5A RAB5
P20339,Q9CQD1
Rabbit
Polyclonal
Rabbit IgG
Endocytosis #Phagocytosis
Product Information
| Description | Rab5rabbit polyclonal |
| Protein full name | Ras-related protein Rab-5A |
Synonyms | Rab5a, nnyRab5a, Ubiquitin-protein ligase D3 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human Rab5 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm,Cell membrane |
Predicted MW. | 24 kDa |
Observed MW. | 24 kDa |
| Uniprot ID |
Applications
| Applications | WB | IHC | IF |
| Species | Human,Mouse,Rat | Human | Human |
| Dilution | 1:500-1:1000 | 1:1000-1:2000 | 1:500-1:1000 |
| Positive tissue | brain, testis | lung, lung cancer | lung, lung cancer |
Background
Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes, and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.
Images
| Western blot analysis of Rab5 (T611104M) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embeddehuman lung using Rab5 (T611104M) at dilution of 1:2000 |
| Immunohistochemistry analysis of paraffin-embedded human lung cancer using Rab5 (T611104M) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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