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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability. .
Polyadenylate-binding protein 1 (PABP-1) (Poly(A)-binding protein 1) Pabpc1 Pabp1
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
71kD
Pabpc1 Pabp1
P29341,Q9EPH8
Rabbit
Polyclonal
Rabbit IgG
Product Information
| Description | PABPrabbit polyclonal |
| Protein full name | Polyadenylate-binding protein 1 |
Synonyms | PABP-1, Poly(A)-binding protein 1, Pabpc1, Pabp1 |
| Immunogen | Recombinantproteincorrespondingto Mouse PABP |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | |
Predicted MW. | 71 kDa |
Observed MW. | 71 kDa |
| Uniprot ID |
Applications
| Applications | WB | IHC | IF |
| Species | Human,Mouse,Rat | Mouse,Rat | Mouse,Rat |
| Dilution | 1:500-1:1000 | 1:1000-1:2000 | 1:500-1:1000 |
| Positive tissue | liver, testis, brain | testis | testis |
Background
PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
Images
| Western blot analysis of PABP (T611164M) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse testisusing PABP (T611164M) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin-embedded rattestisusing PABP (T611164M) at dilution of 1:1200 |
| Immunofluorescent analysis of paraffin-embedded mouse testisusing PABP (T611164M) at dilution of 1:1200 |
| Immunofluorescent analysis ofparaffin-embedded rattestisusing PABP (T611164M) at dilution of 1:1200 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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