Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Calbindin (Calbindin D28) (D-28K) (Vitamin D-dependent calcium-binding protein, avian-type) CALB1 CAB27
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
31kD
CALB1 CAB27
P05937,P12658,P07171
Rabbit
Polyclonal
Rabbit IgG
Product Information
| Description | Calbindinrabbit polyclonal |
| Protein full name | Calbindin |
Synonyms | Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1 |
| Immunogen | Recombinantproteincorrespondingto Mouse Calbindin |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Nucleus, Cytoplasm |
Predicted MW. | 30 kDa |
Observed MW. | 30 kDa |
| Uniprot ID |
Applications
| Applications | WB | IHC | IF |
| Species | Mouse,Rat | Human,Mouse,Rat | Human,Mouse,Rat |
| Dilution | 1:500-1:1000 | 1:1000-1:2000 | 1:1000-1:2000 |
| Positive tissue | brain, kidney, pituitary | kidney cancer, kidney, brain | kidney, brain, small intestine |
Background
Calbindinis a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.
Images
Western blot analysis of Calbindin (T611211M) at dilution of 1: 1000 | |
| Immunohistochemistry analysis ofparaffin-embedded human kidney cancerusing Calbindin (T611211M) at dilution of 1:2000 | |
| Immunohistochemistry analysis of paraffin-embedded mouse kidney using Calbindin (T611211M) at dilution of 1:2000 | |
| Immunohistochemistry analysis of paraffin-embedded rat kidneyusing Calbindin (T611211M) at dilution of 1:2000 | |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using Calbindin (T611211M) at dilution of 1:2000 | |
| Immunohistochemistry analysis of paraffin-embedded rat brainusing Calbindin (T611211M) at dilution of 1:2000 | |
| Immunofluorescent analysis ofparaformaldehyde-fixedmouse kidney using Calbindin (T611211M)at dilution of 1:2000 | |
| Immunofluorescent analysis ofparaformaldehyde-fixedmouse small intestine using Calbindin (T611211M) at dilution of 1:2000 | |
| Immunofluorescent analysis ofparaformaldehyde-fixedrat brain using Calbindin (T611211M)at dilution of 1:2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱:market@ab-mart.com
应聘职位:hr@ab-mart.com
订购专线:4006-123-828
销售电话:13916964679(微信同号)
技术支持:15618194176(微信同号)
华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:
陆经理:手机13122837132(微信同号)
北方及西南经销商负责:
张经理:手机13122150513(微信同号)

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