T611211

货号 规格 价格(¥)
T611211S 50ul
T611211M 100ul

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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经典一抗-Anti-Calbindin Rabbit pAb

Datasheet

Background

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Alternative Name

Calbindin (Calbindin D28) (D-28K) (Vitamin D-dependent calcium-binding protein, avian-type) CALB1 CAB27

Reactivity

Human,Rat,Mouse

Predicted Reactivity

Application

WB,IHC,IF,ICC

Application Image

Recommended Dilution

WB 1:1000-1:2000
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:100-1:400

Mol Weight

31kD

Gene Symbol

CALB1 CAB27

Uniprot

P05937,P12658,P07171

Uniprot link

Immunogen

Immunogen Range

Source

Rabbit

Clonality

Polyclonal

Isotype

Rabbit IgG

Specificity

Subcellular location

Research Area

Positive control

Conjugation

Modification

Purification

Formulation

Concentration

Buffer

产品介绍

Product Information

Description

Calbindinrabbit polyclonal

Protein full nameCalbindin

Synonyms

Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1

Immunogen

Recombinantproteincorrespondingto Mouse Calbindin

IsotypeIgG
PurityAffinity purification
Subcellular location
Nucleus, Cytoplasm

Predicted MW.

30 kDa

Observed MW.

30 kDa

Uniprot ID

P05937, P12658, P07171


Applications

Applications

WB

IHC
IF
Species

Mouse,Rat

Human,Mouse,Rat

Human,Mouse,Rat
Dilution

1:500-1:1000

1:1000-1:2000
1:1000-1:2000
Positive tissue

brain, kidney, pituitary

kidney cancer,
kidney, brain

kidney, brain,
small intestine

Background

Calbindinis a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.


Images

Western blot analysis of Calbindin (T611211M) at dilution of 1: 1000
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse pituitary tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat kidney tissue lysate
Lane 6: Rat pituitary tissue lysate

Immunohistochemistry analysis ofparaffin-embedded human kidney cancerusing Calbindin (T611211M) at dilution of 1:2000


Immunohistochemistry analysis of paraffin-embedded mouse kidney using Calbindin (T611211M) at dilution of 1:2000


Immunohistochemistry analysis of paraffin-embedded rat kidneyusing Calbindin (T611211M) at dilution of 1:2000

Immunohistochemistry analysis of paraffin-embedded mouse brain using Calbindin (T611211M) at dilution of 1:2000

Immunohistochemistry analysis of paraffin-embedded rat brainusing Calbindin (T611211M) at dilution of 1:2000

Immunofluorescent analysis ofparaformaldehyde-fixedmouse kidney using Calbindin (T611211M)at dilution of 1:2000

Immunofluorescent analysis ofparaformaldehyde-fixedmouse small intestine using Calbindin (T611211M) at dilution of 1:2000

Immunofluorescent analysis ofparaformaldehyde-fixedrat brain using Calbindin (T611211M)at dilution of 1:2000

Storage

StorageStore at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage BufferPBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol.

NOTE: This product is intended for research only.

Clone

Target

波长

Storage

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.

注意事项

Companion Products

Important

Comments

RRID

客户评价

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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