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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
N-terminal EF-hand calcium-binding protein 1 (EF-hand calcium-binding protein 1) Necab1 Efcbp1
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
41kD
Necab1 Efcbp1
Q8BG18,Q9ESB5
Rabbit
Polyclonal
Rabbit IgG
Product Information
| Description | NECAB1 rabbit polyclonal |
| Protein full name | N-terminal EF-hand calcium-binding protein 1 |
Synonyms | EF-hand calcium-binding protein 1, Necab1, Efcbp1 |
| Immunogen | Recombinantproteincorrespondingto Mouse NECAB1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
Predicted MW. | 41 kDa |
Observed MW. | 41 kDa |
| Uniprot ID |
Applications
| Applications | WB | IHC | |
| Species | Mouse,Rat | Mouse,Rat | Mouse,Rat |
| Dilution | 1:500-1:1000 | 1:1000-1:2000 | 1:1000-1:2000 |
| Positive tissue | brain, cerebral cortex, brain, cerebellum | brain, cerebellum | brain |
Background
This protein is the member of the EF-CBP (N-terminal EF-hand calcium binding protein) /NECAB (neuronal calcium-binding protein) family that participate in neuronal calcium signaling. EF-CBP1 (N-terminal EF-hand calcium binding protein 1), also known as STIP-1 or neuronal calcium-binding protein 1(NECAB1), is a 351 amino acid cytoplasmic protein that contains one antibiotic biosynthesis monooxygenase (ABM) domain and two EF-hand domains. Expressed in brain, EF-CBP1 interacts with copine 6 and Syntaxin, and exists as two alternatively spliced isoforms.
Images
| Western blot analysis of NECAB1 (T611242M) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using NECAB1 (T611242M) at dilution of 1:2000 |
| Immunohistochemistry analysis of paraffin-embedded rat brainusing NECAB1 (T611242M) at dilution of 1:2000 |
| Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using NECAB1 (T611242M) at dilution of 1:2000 |
| Immunohistochemistry analysis of paraffin-embedded rat cerebellumusing NECAB1 (T611242M) at dilution of 1:2000 |
| Immunofluorescent analysis of paraformaldehyde-fixedmouse brain using NECAB1 (T611242M) at dilution of 1:2000 |
| Immunofluorescent analysis of paraformaldehyde-fixedrat brainusing NECAB1 (T611242M) at dilution of 1:2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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