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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
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Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability . M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing . Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex (By similarity). The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation . Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs . Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts . Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells . In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation . Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (By similarity). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (By similarity). .
YTH domain-containing family protein 1 (Dermatomyositis associated with cancer putative autoantigen 1 homolog) (DACA-1 homolog) Ythdf1
Mouse
IHC
IHC 1:50-1:200
61kD
Ythdf1
P59326
Rabbit
Polyclonal
Rabbit IgG
Immunity
Product Information
| Description | YTHDF1 rabbit polyclonal |
| Protein full name | YTH domain-containing family protein 1 |
Synonyms | Dermatomyositis associated with cancer putative autoantigen 1 homolog, DACA-1 homolog, Ythdf1 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse YTHDF1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
| Uniprot ID | P59326 |
Applications
| Applications | IHC |
| Species | Mouse |
| Dilution | 1: 1000-1: 2000 |
| Positive Tissue | brain |
Background
YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation.
Images
| Immunohistochemistry analysis of paraffin-embedded mousebrainusing YTHDF1 (T611390M) at dilution of 1:1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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微信客服
邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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