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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis . Induces cartilage and bone formation . Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 . Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A . In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 . Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation . Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation . Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). .
Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A) BMP2 BMP2A
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
45kD
BMP2 BMP2A
P12643,P21274,P49001
Rabbit
Polyclonal
Rabbit IgG
Product Information
Description | BMP2rabbit polyclonal |
Protein full name | Bone morphogenetic protein 2 |
Synonyms | BMP2, BDA2, BMP2A, bone morphogenetic protein 2 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse BMP2 |
Isotype | IgG |
Predicted MW. | 45kDa |
Observed MW. | 45kDa |
Purity | Affinity purification |
Subcellular location | |
Uniprot ID | P12643,P21274,P49001 |
Applications
applications | species | dilution | Positive tissue |
WB | Human, Mouse, Rat | 1:1000 | liver, spleen, lung |
IHC | Mouse, Rat | 1: 200-1:1000 | bone |
IF | Mouse, Rat | 1: 200-1:1000 | bone |
Background
Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Images
| Western blot analysis of BMP2 T61252M) at dilution of 1:1000. Lane1:U251 cell lysate Lane2:Hela cell lysate Lane3:MCF7 cell lysate Lane 4:Mouse liver tissue lysate Lane 5:Mouse spleen tissue lysate Lane 6:Mouse lung tissue lysate Lane 7:Rat liver tissue lysate Lane 8:Rat spleen tissue lysate Lane 9:Rat lung tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded mouse bone marrow using BMP2(T61252M) at dilution of 1:500 |
 | Immunohistochemistry analysis of paraffin-embedded mouse bone using BMP2(T61252M) at dilution of 1:500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
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