13916964679
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis . Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins . At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond . Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) . Cleaves and inactivates interleukin-18 (IL18) . Involved in the cleavage of huntingtin . Triggers cell adhesion in sympathetic neurons through RET cleavage . Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress . Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction . Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) . Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface . .
Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] CASP3 CPP32
Human,Rat,Mouse
IHC,IF,ICC
IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
32kD
CASP3 CPP32
P70677,P42574,P55213
Rabbit
Polyclonal
Rabbit IgG
Apoptosis #DNA damage
Product Information
Description | Cleaved- Caspase-3 rabbit polyclonal |
Protein full name | Caspase-3 |
Synonyms | CASP3, CPP32, CPP32B, SCA-1, Caspase 3, Caspase-3 subunit p12, PARP cleavage protease |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse caspase3 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
IHC | Human, Mouse, Rat | 1: 500-1: 1000 | mouse tumor, A549 mice tumor, MCF7 mice tumor |
IF | Human | 1: 500-1: 1000 | esophagus, stomach, colon |
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Images
 |  |
Immunohistochemistry analysis of paraffin-embedded A549 in mice tumor using Cleaved- Caspase-3(T61532M) at dilution of 1: 1000, untread (left), treated with the control peptide antigen (right) . | |
 |  |
Immunohistochemistry analysis of paraffin-embedded MCF-7 in mice tumor using Cleaved- Caspase-3 (T61532M) at dilution of 1: 1000, untread (left), treated with the control peptide antigen (right) . | |
 |  |
Immunohistochemistry analysis of paraffin-embedded mouse tumor using Cleaved- Caspase-3 (T61532M) at dilution of 1: 1000, untread (left), treated with the control peptide antigen (right) . | |
| |
| Immunofluorescent analysis of paraformaldehyde-fixed human esophagus using Cleaved-Caspase3 (T61532M) at dilution of 1:500 . | |
| |
| Immunofluorescent analysis of paraformaldehyde-fixed human stomach using Cleaved-Caspase3 (T61532M) at dilution of 1:1000 . | |
| |
| Immunofluorescent analysis of paraformaldehyde-fixed human colon using Cleaved-Caspase3 (T61532M) at dilution of 1:500 . | |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
艾比玛特医药科技（上海）有限公司
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微信客服
邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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