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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Green fluorescent protein GFP
General
WB,IF,ICC
WB 1:1000-1:2000IF 1:100-1:400ICC 1:100-1:400
27kD
GFP
P42212
Rabbit
Polyclonal
Rabbit IgG
Luminescence #bioluminescence
Product Information
Description | EGFP rabbit polyclonal |
Protein full name | Enhanced GFP |
Synonyms | Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP,Enhanced Green Fluorecent protein, eGFP, EGFP |
Immunogen | KLH conjugated Synthetic peptide corresponding to MouseeGFP |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 27 kDa |
Observed MW. | 29 kDa |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human,Rat, Mouse, Tag | 1:500-1:1000 | cells/ tissues with eGFP |
IF | Human,Rat, Mouse, Tag | 1: 500-1: 1000 | 293T |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining. Other applications of eGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
Images
 | Western blot analysis of eGFP (T61602M) at dilution of 1: 500 Lane 1: 293T cell lysate Lane 2: 293T with eGFP cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 with eGFP cell lysate |
 | Immunofluorescence analysis of human 293T cells using eGFP (T61602M) at dilution of 1:1000 A: DAPI nuclear stain ( blue ) B: eGFP protein ( green ) C: Anti-eGFP ( red ) D: Merged |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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微信客服
邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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