Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Calponin-1 (Basic calponin) (Calponin H1, smooth muscle) Cnn1
Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
34kD
Cnn1
Q08091,Q08290
Rabbit
Polyclonal
Rabbit IgG
Product Information
| Description | Calponin 1rabbit polyclonal |
| Protein full name | Calponin-1 |
Synonyms | Basic calponin, Calponin H1, smooth muscle, Cnn1, Calponin 1 basic smooth muscle, Calponins basic, Sm Calp, SMCC |
| Immunogen | KLH conjugated Synthetic peptidecorresponding to MouseCalponin 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
Predicted MW. | 33 kDa |
| Observed MW. | 33 kDa |
| Uniprot ID |
Applications
| Applications | WB | IHC | IF |
| Species | Mouse,Rat | Mouse,Rat | Mouse,Rat |
| Dilution | 1:500-1:1000 | 1:500-1:1000 | 1:500-1:1000 |
| Positive tissue | uterus, blood vessels, stomach, lung | uterus | uterus |
Background
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is expressed in smooth muscle and tissues containing significant amounts of smooth muscle. Two isoforms of calponin exist whose molecular weights are 34kDa and 29kDa.
Images
![]() | Western blot analysis of Calponin 1 (T61954M) at dilution of 1: 1000 Lane 1: Mouse uterus tissue lysate Lane 2: Mouse blood vessels tissue lysate Lane 3: Mouse stomach tissue lysate Lane 4: Mouse lung tissue lysate Lane 5: Rat uterus tissue lysate Lane 6: Rat stomach tissue lysate | |
![]() | Immunohistochemistry analysis of paraffin-embedded mouse uterususingCalponin 1 (T61954M)at dilution of 1:1000 | |
![]() | Immunohistochemistry analysis of paraffin-embedded rat uterususingCalponin 1 (T61954M)at dilution of 1:1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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订购专线:4006-123-828
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程经理:手机18616261485(微信同号)
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徐经理:手机15618191473(微信同号)
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