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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
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Synthesis and degradation of fructose 2,6-bisphosphate. .
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (6PF-2-K/Fru-2,6-P2ase 2) (PFK/FBPase 2) (6PF-2-K/Fru-2,6-P2ase heart-type isozyme) (RH2K) [Includes: 6-phosphofructo-2-kinase (EC 2.7.1.105); Fructose-2,6-bisphosphatase (EC 3.1.3.46)] Pfkfb2
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
65kD
Pfkfb2
P70265,Q9JJH5
Rabbit
Polyclonal
Rabbit IgG
Glycolysis
Product Information
| Description | PFKFB2rabbit polyclonal |
| Protein full name | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
Synonyms | PFKFB2, PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 |
| Immunogen | RecombinantproteincorrespondingtoMouse PFKFB2 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm,Nucleus |
Predicted MW. | 60 kDa |
| Observed MW. | 64 kDa |
| Uniprot ID | P70265,Q9JJH5 |
Applications
| Applications | WB | IHC | IF |
| Species | Human,Mouse | Mouse,Rat | Mouse,Rat |
| Dilution | 1:500- 1:1000 | 1:500-1:1500 | 1:250-1:500 |
| Positive tissue | kidney, heart | lung, brain | lung, brain |
Background
The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation . Four different PFKFB isoforms (PFKFB1, PFKFB2,PFKFB3, and PFKFB4) have been identified. Research tudies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2. In addition, androgen increases the expression of PFKFB2 in prostate cancer cells.
Images
 | Western blot analysis of PFKFB2(T61989M) at dilution of 1: 1000 Lane 1: 293T cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: Mouse kidney tissue lysate Lane 4: Mouse kidney tissue lysate Lane 5: Rat Heart tissue lysate Lane 6: Rat Heart tissue lysate | |
 | Immunohistochemistry analysis of paraffin-embedded mouse lungusingPFKFB2 (T61989M) at dilution of 1:1000 | |
 | Immunohistochemistry analysis of paraffin-embedded rat lung usingPFKFB2(T61989M) at dilution of 1:1000 | |
 | Immunohistochemistry analysis of paraffin-embedded mouse brain usingPFKFB2(T61989M) at dilution of 1:1000 | |
 | Immunohistochemistry analysis of paraffin-embedded rat brain usingPFKFB2(T61989M) at dilution of 1:1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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微信客服
邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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