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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells . Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction . In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA . Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). .
Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed] ACTB
Human,Rat,Mouse
WB,IHC,IF,ICC
WB 1:1000-1:2000IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
42kD
ACTB
P60709
Mouse
Monoclonal
Mouse IgG
PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.
Product Information
| Description | beta Actinmouse monoclonal |
| Protein full name | Actin, cytoplasmic 1 |
Synonyms | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta,Beta CytoskeletalActin |
| Immunogen | KLH conjugated Synthetic peptidecorresponding to Mouse beta;-Actin |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW. | 42 kDa |
| Observed MW. | 45 kDa |
| Subcellular location | Cytoplasm |
| Uniprot ID | P60709,P60710,P60711 |
Applications
| Applications | WB | IHC,IF | |
| Species | Human,Rat,Mouse | Human,Rat,Mouse | |
| Dilution | 1:1000-1:2000 | 1:400-1:2000 | |
| Positive tissue | Hela,Raw264.7,liver,brain | Liver,lung,stomach |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
 | Western blot analysis of beta;-Actin (T62001M) at dilution of 1:1000. Lane 1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:C6 cell lysate Lane4:HCT116 tumor tissue lysate Lane5:HepG2 tumor tissue lysate Lane6:Mouse liver tissue lysate Lane7:Mouse brain tissue lysate Lane8:Rat liver tissue lysate Lane9:Rat brain tissue lysate |
 | Immunohistochemistry analysis of paraffin-embedded human liver using beta;-Actin(T62001M) at dilution of 1:1300 |
 | Immunohistochemistry analysis of paraffin-embedded mouse ovary using beta;-Actin(T62001M) at dilution of 1:400 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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