13916964679
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Human,Rat,Mouse
IHC,IF,ICC
IHC 1:50-1:200IF 1:100-1:400ICC 1:100-1:400
n/a
Mouse
Monoclonal
Mouse IgG
Product Information
| Description | BrdU mouse monoclonal |
| Protein full name | Bromodeoxyuridine |
| Synonyms | Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2prime;-deoxyuridine; 5-BrdU,Proliferation Marker; 5-Bromo-2-deoxyUridine |
| Immunogen | KLH conjugated Synthetic peptide corresponding to MouseBrdU |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Nuclear |
| CAS: | 59-14-3 |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC | Human, Mouse, Rat | 1: 100-1: 500 | Jejunum |
| IF | Human, Mouse, Rat | 1: 100-1: 500 | Hela |
Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.
Images
 | Immunofluorescence analysis of Hela cells（treated with 0.03mg/ml BrdU for 40 min )using BrdU (T62051M) at dilution of 1:100 |
 | Immunofluorescence analysis of Hela cells（treated with 0.03mg/ml BrdU for 2 h )using BrdU (T62051M) at dilution of 1:100 |
 | Immunohistochemistry analysis of paraffin-embedded mouse jejunum(intraperitoneal injection BrdU every 2 hours for 4 times,the mouse were killed after 24 hours and the jejunum were fixed with 4%PFA)using BrdU (T62051M) at dilution of 1:300 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
微信客服
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