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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. .
Tubulin beta-3 chain (Neuron-specific class III beta-tubulin) Tubb3
Human,Rat,Mouse
WB,IF,ICC
WB 1:1000-1:2000IF 1:100-1:400ICC 1:100-1:400
51kD
Tubb3
Q9ERD7,Q4QRB4
Mouse
Monoclonal
Mouse IgG
Product Information
| Description | beta III Tubulin mouse monoclonal |
| Protein full name | Tubulin beta-3 chain |
Synonyms | TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III |
| Immunogen | KLH conjugated Synthetic peptidecorresponding to Mouse beta;3-tubulin |
| Isotype | IgG |
| Purity | Affinity purification |
Predicted MW. | 55kDa |
Observed MW. | 55kDa |
| Subcellular location | Cytoplasm |
| Uniprot ID |
Applications
| Applications | WB | IF |
| Species | Human, Mouse,Rat | Mouse |
| Dilution | 1:1000-1:2000 | 1: 500-1: 1000 |
| Positive tissue | brain, glioma,cortex, hippocampus | Neuro-2a |
Background
Cysteine residues in class III beta;-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III beta;-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.
Images
 | Western blot analysis of beta;3- tubulin(T62139M) at dilution of 1: 1000 Lane 1: U87 cell lysate Lane 2: Human brain tissue lysate Lane 3: Human glioma tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse cortex tissue lysate Lane 6: Mouse hippocampus tissue lysate Lane 7: Rat brain tissue lysate Lane 8: Rat cortex tissue lysate Lane 9: Rat hippocampus tissue lysate | |
 | Immunofluorescence analysis of human Neuro-2a cells using beta;3- tubulin (T62139M)at dilution of 1: 1000 A: DAPI nuclear stain ( blue ) B: Anti-eGFP ( red ) C: Merge |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 mu;g/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13916964679（微信同号）
技术支持：15618194176（微信同号）
华南经销商负责（广东，广西，福建，海南）：
程经理：手机18616261485（微信同号）
华北经销商负责（北京，天津，河北）：
徐经理：手机15618191473（微信同号）
南方经销商负责：
陆经理：手机13122837132（微信同号）
北方及西南经销商负责：
张经理：手机13122150513（微信同号）
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